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For a further modification of DH-13, 2,1,3-benzothiadiazole has been inserted to construct a D-A-π-A structured dye (DH-16) which achieves a power efficiency of 6.76% without coadsorbent that reached 89% of the reference dye N719-based cell.
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To provide good coverage and potentially include complex biofuel tolerance mechanisms that involve multiple genes we used relatively large (~40 kb) inserts to construct a fosmid library, which we screened in E. coli.
Full length RAGE was inserted into a pCDNA3.1 vector to construct a mammalian expression plasmid (PCDNA3.1-RAGE).
These two fragments were ligated and inserted into the plasmid pYX242 to construct a vector, pYX242-WS, with two sites that can be used to express genes.
The human C/EBPα CDS exons plus 3′-UTR were inserted into the pCDNA3.1 vector to construct a human C/EBPα-overexpression plasmid pCDNA-C/EBPα, which could be inhibited by miRNAs (such as miR-381).
The human Cx43 CDS exons plus 3′-UTR were inserted into the pCDNA3.1 vector to construct a human Cx43 overexpression plasmid pCDNA-Cx43, which could be inhibited by miR-200a via the 3′-UTR.
Full-length mRFP amplified with primers 5′-TTGCCGCTGCGGCTGCCTCCTCCGAGGACGTC-3′ and 5′-CCTAGCTAGCGGCGCCGGTGGAGTGGC-3′ was inserted between BstAPI and SpeI sites to construct a pFlex-mRFP plasmid.
The random DNA fragments, generated from mRNAs of Saccharomyces cerevisiae using a novel "DNA random priming method", were inserted into a surface display vector to construct a combinatorial random protein library.
We show that using a fosmid vector (insert size between 30 40 kb) to construct a genomic library (480 clones), we can recover the whole 82 kb integron array in four PCR screening rounds, the first of which identifies a clone bearing the intI.
Briefly, total genomic DNA (nuclear, mitochondrial and chloroplast) was used to construct a large insert fosmid library.
HMW DNA of 1.8 million nuclei (∼5.4 µg DNA) was used to construct a large insert library cloned in pIndigoBAC-5 vector (Epicentre, Madison, WI, USA) as previously described.
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