Similarly, the cDNA of mKate (kindly provided by Dmitriy Chudakov, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, MoscowasRussiamplifiedplified with PCR primers 5'-ATC andGGTACCATGAGCGAGCTGATCAAG-3' and 5'-ACT CTCGandCTTGTGCCCCAGTTTGC-3' and inserted to obtain pmKate loxP.
Pesticide resistant crops have had the toxic gene for Bt (Bacillus thuringiensis, a naturally occurring bacterium that causes disease in insects) inserted into them to act as a built-in pesticide.
The cathedral in the city's central square, known as the Zócalo, famously sunken in spots during the last century, is a kind of fun house, with a leaning chapel and a bell tower into which stone wedges were inserted during construction to act more or less like matchbooks under the leg of a wobbly cafe table.
At each grid junction, they inserted 1,000 molecules to act as switches.
We inserted an endoscope to act as a stent during transverse closure of the esophagus and performed a leak test that was negative (Fig. 2d).
The gene circuit from gene j to gene i can be implemented by inserting the binding site of gene product j (i.e. the protein of gene j) into the promoter region of gene i so that the protein of gene j could bind this inserted binding site to act as a transcription factor (TF) to regulate the gene expression of gene i.
Once the animals were anaesthetized, hypodermic needles were inserted through the skin to act as recording electrodes over the right and left mastoids, and a reference needle was inserted at the vertex point.
Carbon or basalt fiber cores were embedded in SFRP composites as core reinforcements, and of which their ends were inserted into the beams to act as anchors.
A trigonal graphene [17], biphenyl molecule [18], carbon atomic chains [19], single-molecule magnet Fe4 [20] are inserted between two GNRs to act as a spin filter.
This plasticine was pressed into the cast, along with a black cable tie inserted into the model to act as an anchor point.
