Sentence examples for inserted site from inspiring English sources

Meanwhile, since the inserted gene is fused with viral nonstructural protein gene, the open reading frame of the viral coding region downstream of the inserted gene should not be changed after the insertion; the viral gene-encoded protein sequence downstream of the inserted site should not be interrupted.

Previously, we studied the exonization effect of a single inserted site by Ds providing 5 splice donor sites (1 for forward and 4 for reverse pattern), which may result in 5 exonized transcripts.

The second reaction with primers (0168-1/0168-2 of pD1-1/p pD1-2d-2) of inserted site verified the wild-type was replaced completely.

We attempted the 5′RACE method first because the mRNA upstream of the inserted site was expected to be trapped by the splice acceptor localized at the beginning of the gene trap vector.

When we measure the amount of mRNA of A61, we used primers and probes for real-time PCR at both upstream (5′ primers in Fig. 4) and downstream of the inserted site (3′ primers in Fig. 4) and found a reduction of mRNA by both primers and probes (Fig. 6 A).

We could not find splicing from the upstream exons (exons 1 and 2) to the splice acceptor of the gene trap vector (Fig. 6 B), which suggests that the reduced amount of mRNA in the upstream of the inserted site was not because of the skipping of the upstream probe located at exons 3 and 4. In these cell lines, two possibilities were considered for their phenotypes.

The designed YS nanostructure with the large amount of nanosheets on its shell increased the inserted sites of Na ions due to large surface area.

However, tools to insert site-specific methylation into DNA for analyzing epigenetic functions are limited.

The coding regions for viral nonstructural proteins, especially for the proteins with multistructural domains, are commonly selected to be the candidate inserting sites for exogenous genes.

We compared these candidate insert sites to known polymorphic L1s and Alus [ 13, 25, 26], and about 1%63(63 out of approximately 5300) were within 1kb, without considering direction of read.

They use the combined activities of an autocatalytic intron RNA (a "ribozyme") and an intron-encoded reverse transcriptase (RT) to insert site-specifically into DNA with high efficiency and high and readily programmable DNA target specificity.