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Three kinds of linear PCR templates were generated: (i) a C-terminal wild type with C-terminal 6xHis-tag (ii) a C-terminal mutant with C-terminal 6xHis-tag and inserted silent mutation at the N-terminus (iii) a N-terminal wild type with N-terminal 6xHis-tag and no attachment sites (att-sites); For the C- and N-terminal wild type template the ORF is identical to the original ORF in the plasmid DNA.
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Additionally, we inserted a silent gap of 3 s and we copied both the gap and the strophe to append them several times in order to create a 2 minutes loop song.
The ten motifs chosen from the familiar songs had a duration of 575 ms±17 mean±SDn±SD), and the ten motifs from the unfamiliar songs had a duration of 555 ms±29 ms. For creating the gap motifs we inserted a silent gap with a duration of 50 ms every 75 ms (Fig. 1).
The expression of GFP gene inserted at the silent HMR locus (hmr::GFP) was used to monitor the degree of silencing by quantifying the percentage of yeast cells expressing GFP (Fig. 1A).
UV-mutagenesis was performed on a strain containing different marker genes inserted at various silent chromatin domains: the central core of centromere 1 (cnthearg3+), the outer repeat of centromere 2 (otrespectivelyespectively, and a telomere (tel1L:his3+) (Figure 1A) [22].
We constructed derivatives of Typhimurium strain 14028s containing a kanamycin resistance cassette (kanR) inserted in a genetically silent region downstream from the spv operon in the virulence plasmid.
To rule out such a bias as an explanation of increased fused reports for words, we also required listeners to detect an additional silent gap inserted after the initial /s/ of a minority (6%) of syllables.
Intriguingly, he sometimes adds sound because one of the things you notice on digital formats is silence – the incongruously absolute silence, for instance, of silent "leader" tape inserted between movements of a classical symphony, as many engineers used to do.
Each song was played for approximately 2 min and a 16 s silent rest was inserted between each musical excerpt to reduce any influence of the previous song.
Growth for several cell cycles in the presence of the HDAC inhibitor Trichostatin A (TSA) induced hyperacetylation and transcription in normally silent test genes inserted into centric heterochromatin.
One change was a a missense variant in exon 9 and the second change a silent variant that inserted a putative exonic enhancer site in exon 13.
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