Sentence examples for inserted mutation from inspiring English sources

Thus, the phenotypes are considered to correctly reflect the effects of the inserted mutation.

Prediction of the secondary structure of CCTα apical domain in wildtype and in the G346E mutant cells was done using the program ProModII [31], since the inserted mutation was in this protein domain.

Figure 2D shows that the inserted mutation at −774 bp of the β-syn promoter, corresponding to the putative MTF-1 binding site, abolishes promoter activity to near basal levels, regardless of MTF-1 over-expression, as can be seen when compared to pGL3basic.

This result suggested that I-SceI failed to cleave at its target site in the inserted mutation cassette.

Although the TAG content increased, growth kinetics of the starchless C. reinhardtii strains are severely subdued by the inserted mutation [ 20], resulting in decreased TAG productivity [ 11].

We discovered that the intransigence of certain genes was due to active transcription that continued into the inserted mutation cassette and prevented cleavage at the I-SceI site.

Then, E. coli JM109 cells were transformed with the plasmid pPR-pncrA or pPR-pncrB alone, or co-tranformed with the plasmid pNTA, pNTB, or pNTC (which contained the inserted mutations in ncrA, ncrB, or ncrC by tetracycline box insertion) [3].

In line with previous observations, expressions of fCJD PrPs, including point-mutations and inserted mutations in octarepeats, can cause clear cytotoxicity on the cultured cells[30].

Here we have taken an unbiased genetic approach to identify functionally important domains of Munc18-1, utilising randomly inserted mutations in the Munc18-1 structure.

Genetic prion diseases are linked to point and inserted mutations in the prion protein (PrP) gene that are presumed to favor conversion of the cellular isoform of PrP (PrPC) to the pathogenic one (PrPSc).

Based on this wild-type genomic nbr sequence, we inserted 1 ×  HA tag sequence at the C-terminus, as well as inserted mutations (D435A,E437A) in the exonuclease domain by additional PCRs.