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A 3 x influenza HA epitope tag was inserted into the expression vectors at the position corresponding to the C terminus of the expressing protein.
The synthetic mammalian tandem repeated GnRH hexamer gene was inserted into the expression plasmid pMAL-c2x.
The PCR product was restriction digested with FseI and AscI and inserted into the expression vector pLN-Ty1C44.
The results indicated that PLFN gene had inserted into the expression vectors and achieved multiple Lactobacillus expression systems.
2,3-BD biosynthesis gene clusters were cloned from several native 2,3-BD producers, including Bacillus subtilis, Bacillus licheniformis, Klebsiella pneumoniae, Serratia marcescens, and Enterobacter cloacae, inserted into the expression vector pET28a, and compared for 2,3-BD synthesis.
In this experiment, we used the genetic engineering method, fusion PCR technique, to connect methionine-rich gene (zein) from maize endosperm protein with lysine-rich gene (Cflr) from the pepper anther, then the fusion gene was inserted into the expression vector pHT43, and the recombinant plasmid pHT43/zein-Cflr was constructed.
Similar(25)
In the study of Zhao et al. [ 31], the amplified CXCL10 gene was inserted into the eukaryotic expression vector pcDNA3.1, and the recombinant expression vector pcDNA3.1-IP-10 pcDNA3.1-IP-10 pcDNA3.1-IP-10cells via liposome.
For expression of FLAG epitope tagged ANT, the cDNAs were inserted into the mammalian expression vector pCMV-tag2.
The corresponding NtMMP1-apo and NtMMP1-KDEL cDNAs were inserted into the plant expression vector pTRAkt and the proteins transiently expressed in tobacco leaves.
MicroRNA sequences were inserted into the pSM30 expression vector which provides enhanced green fluorescent protein as an indicator of microRNA expression.
For expression of catalase A with a carboxyl-terminal HA epitope, the cDNA was inserted into the yeast expression vector pRS424 carrying the TFA1 promoter.
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