The neonatal rat cardiomyocytes were inserted into a thermostatic (37°C) cuvette containing 2 mL of calcium-free PSS.
To generate HA (haemagglutinin)–YFP (yellow fluorescent protein)–Munc18b transgenic mice, the HA epitope tag and Venus YFP were cloned by PCR in tandem with rat Munc18b and inserted into a mammalian expression vector containing the mouse CCSP (Clara cell secretory protein) promoter, a rabbit β-globin intron, and the bovine growth hormone polyadenylation signal [ 34, 35].
The electrodes were inserted into a silicone chamber which was used to bridge a gap in a rat sciatic nerve.
Rats were anaesthetised, a butterfly catheter inserted into a tail vein for contrast agent administration, and placed supine into a 63 mm quadrature birdcage coil.
Each scaffold implant construct was inserted into a surgical pocket in triplicate for each assay (using 3 different rats).
To generate these cRNA probes, cDNA fragments of rat Smad4 (268 bp), or GAPDH (597 bp) genes were inserted into a pCRII-TOPO vector.
An injector needle was then inserted into the rat jugular vein, through which 0.025, 0.05, 0.1 and 0.2 mg/kg body weight methacholine (O-Acetyl-b-methylcholine chloride, MCH, Sigma, USA) were injected successively at a 5 min-intervals.
The tip of the pipette was inserted into the rat vagina, but not deeply to avoid cervical stimulation.
These sources were inserted into the rat (mouth, neck, shoulder × 2, lung × 2, liver, right kidney, intestine, bladder and back × 2) by surgery.
Since the sources inserted into the rat were isolated well, it is possible to segment sub-volumes for individual sources on the reconstructed SPECT image.
