The infusion cannula in our experiment was trocar inserted through intact conjunctiva, with no suturing to the sclera, because most vitrectomies are now so performed.
Following small skin incisions, these pins are inserted into intact bone on both sides of a fracture, allowing the external structure to hold and stabilize bone alignment.
Assembly of copious NPCs is also initiated on chromatin, but whether NPCs assemble and are inserted into intact NE sheets or are rather enclosed by the re-forming NE remains controversial.
Briefly, at the HIS3 locus of chromosome XV was inserted an intact URA3 marker flanked by 3′- and 5′-truncated fragments of HIS3 gene that share 223 bp of homology.
By inserting an intact copy of the gene into tomatoes, the researchers increased the amount of glucose and fructose in ripe fruits by up to 40%, the authors write in the paper, published online today in Science.
The inserts caused no adverse effects on viral infectivity and virulence, and the inserted sequences remained intact in progeny viruses in the systemically infected leaves.
Recombinant Ad genomes were constructed to encode a CMV promoter/firefly luciferase expression cassette in the E1 region, the 566FF-R-R34 or 566FF-EE12RR345L fiber and the complementary zipper-scFv G28-5 intorthe intacthE3intact E3 region in the place of the deleted 19 kDa protein (Fig. 1D).
All of the inserted implants remained intact without any complication in their original position during and after the experiment.
The dendrogram shows that the intact (inserted) rarA are almost identical to each other in the BoNT/E-producing C. botulinum and C. butyricum strains, suggesting a common source.
For an NPC to be inserted into the intact NE, both the inner and the outer NE membranes must approach at a site and fuse to give rise to the pore membrane, upon which the core scaffold and the rest of the NPC can then assemble.
