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These peptide sequences were successfully inserted individually as well as all together in a multivalent strategy.
In the first series, one, three, five and seven blastomeres were biopsied from 2-, 4-, 6- and 8-cell embryos respectively, and inserted individually into empty ZP recipients, leaving the remaining blastomere within its original ZP.
Each oligonucleotide tag was inserted individually into the blunted KpnI site of p TnMod-OΩ (Fig. 1).
The expression cassettes were excised with the restriction enzyme HindIII and inserted individually into the binary vector pCB301-Kan [ 51], resulting in the plant expression vectors (VSO1)n-100xELP-pCB301-Kan.
Trees can be made from bits of Woodland Scenics ground foam, and inserted individually.
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To clone the genes, we designed primer pairs to amplify each open reading frame by PCR and inserted them individually into pXDC61M to generate translational fusions with the upstream β-lactamase gene (Fig. 1A).
In the torodial pore mechanism, membrane disruption occurs as polycations insert individually.
Another showed packets of ballots apparently just removed from the ballot box, stacked neatly, one on top of the other, an indication that they were inserted together, not individually.
These three DNA fragments with overlapping sequences were individually inserted into the pMD18-T vector (Takara, http://www.takara.com.cn/) to produce the 7179 bp fragment.
The XbaI XhoI fragment of FeaB, AldB or AldH from pDG3, pDG4 or pDG5 was individually inserted into NheI and XhoI sites of pDG30 to give pDG6, pDG7 and pDG8.
Equal weights of each film were individually inserted in test tubes, each containing 10 ml of sterile Mueller Hinton (MH) broth (composed of g/l: beef extract, 2.0; casein hydrolysate, 17.5 and starch 1.5; pH 7.3 ± 0.2).
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