Exact(7)
By combining the results from the single carrier insertions, we have expressed functional "double fusion" proteins containing cytochromeb562 domains inserted in two different loop positions.
A commercial composite resin (Z350, 3 M ESPE, St Paul, MN, USA) was inserted in two increments of 2 mm and photocured for 40 seconds each to simulate tooth restoration.
Although working with such a large clone was not straightforward, gfp was successfully inserted in two places.
In the M. anisopliae strains where pr1J had been sequenced previously, it was suggested an intron was inserted in two strains, rather than the other strain losing an intron [ 50].
Both parts were inserted in two steps into mCitrine-C1 that was modified with an additional FseI restriction site by mutagenesis PCR with primers mCitrine-FseI FP and mCitrine-FseI-RP.
Full length S. cerevisiae TBCC, TBCD, TBCE, and Arl2 cDNAs (also named Cin2, Cin1, Pac2, and Cin4, respectively) were amplified by PCR using oligonucleotides and inserted in two polycistronic bacterial expression vectors using isothermal assembly and confirmed by DNA sequencing.
Similar(53)
The Ontario government began a major advertising campaign in the spring, including a 16-page color brochure inserted in eight upscale American magazines like Vogue, Architectural Digest and Gourmet.
In the Yale study, 53.8percentt of the subjects who had needles inserted in four acupuncture "zones" in the ear five times a week tested free of cocaine at the end of the eight-week study period.
Mr Freedman writes of the furore in one Los Angeles congregation aroused by a woman who, leading services, inserted in one of the most sacred prayers the names of the four matriarchs alongside those of the three patriarchs.
A single proximal screw was inserted in nine patients, while two screws were inserted in the remaining two.
Nineteen middle frequency sequences inserted in three and a half low frequency transgression/regression cycles were evidenced.
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