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Firstly, the membrane resistance decreases if a quaternary ammonium group or a carboxyl group is inserted in the intermediate layer instead of a secondary or tertiary amino group, which implies that the water dissociation occurs not only due to the catalytic effect of the secondary or tertiary amino group but also to the electrical field.
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The resulting SPg-Xylanase fragment was inserted in the binary vector pCZera-ECFP [25] by SalI/AscI digestion to obtain the intermediate pCSPXyl-ECFP.
After intermediate cloning in the pBSK+ vector and re-sequencing, the fragment was inserted in the pBI101 vector [ 21] upstream of the GUS reporter gene, resulting in the plasmid pBI33 GUS.
They are inserted in the structure equations.
It will be inserted in the document.
If the PPT is not followed by the correct signal for INT processing, the cDNA intermediate will be unable to insert in the new genomic location.
After intermediate cloning in the pBSK+ vector and re-sequencing, the fragment was inserted, in antisense orientation, instead of the GUS gene in the pBI33 GUS vector, resulting in the plasmid pBI33: AS-e (Fig. 2).
After intermediate cloning in the pBSK+ vector and re-sequencing, the fragment was inserted, in antisense orientation, to replace the GUS gene in the pBI33 GUS vector [ 3].
The lenses are inserted in New York.
For the NAF1-containing intermediate complex, N-terminal HA-tagged NAF1 was inserted in place of HA-GAR1.
Double-slash indicates additional resistance genes and mobile elements inserted into the proposed intermediate structures.
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