Sentence examples for inserted in the expression from inspiring English sources
The 3′ end of Lcy DNA segment was connected together by an intron to inform the RNA interferential segment which was then inserted in the expression vector with the Pds promoter to inform the fruit-specific expression vector.
Using similar approach, an antisense sequence to miR-328 was inserted in the expression vector producing an anti-miR-328 construct.
The sequences of the genes inserted in the expression vectors were confirmed on a CEQ 8000 sequencer (Beckman Coulter, Carlsbad, CA, USA).
LPCAT3-null cells were established by transfecting a pair of single guide RNAs, inserted in the expression vector pX459 (Addgene, plasmid 48,139).
In order to construct the plasmid vectors for overexpression of the genes in T. reesei, the PCR fragments were inserted in the expression vector pMS204 using the Gateway recombination system (One-Tube protocol) according to the manufacturer's instructions (Invitrogen, Carlsbad, California, USA).
To generate prey-tagged expression vectors used in co-immunoprecipitation assay or immunofluorescence, the coding regions of various genes were amplified by PCR using as template the corresponding EST clones obtained from RZPD (Berlin, Germany) and then inserted in the expression vectors using Gateway technology (Invitrogen, Carlsbad, CA).
Expressions for the longitudinal and transversal Green's function are presented that can be inserted in the general expression to solve a wide variety of transport problems in infinite, semi-infinite, and finite media.
The rat-mouse 20D5HCmIgG1-Q cDNAeric cDNA sequences were inserted in the pEE14.4 expression vector provided by Lonza Biologics.
The DNA sequence was inserted in the bacterial expression vector pET21 (Novagen).
The aurora-A encoding sequence was inserted in the bacterial expression vector pET21 (Novagen) as previously described (21).
Human MXI1 cDNA was amplified by RT PCR on lymphocyte RNA and inserted in the eukaryotic expression plasmid pCR3, under the control of the CMV promoter.
