The needle was inserted in-plane from lateral to medial and advanced toward the lateral aspect of the femoral nerve.
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The needle and sheath were horizontally inserted in the breast and advanced towards the lesion.
This may be achieved with two cannulae, typically inserted in the femoral vein, and advanced into the inferior vena cava (IVC), and the internal jugular vein, advanced into the SVC.
For measurement of aortic pressure, a fluid filled polyethylene catheter was inserted in the right carotid artery, advanced into the aortic arch and connected to a pressure transducer.
Dilatation of the lower punctum was performed and a 20G vitreoretinal probe was inserted in the lower canaliculus and advanced into the nasolacrimal sac.
Mice were anaesthetized with isoflurane, the left carotid artery was isolated and the tip of the telemeter catheter (transmitter model TA11PA-C10) was inserted in the carotid artery and advanced in the aortic arch, with the telemeter body positioned in a subcutaneous pocket on the right flank.
An arterial catheter was surgically inserted into the internal carotid artery and a 5-French Edwards Swan-Ganz catheter (Baxter Healthcare Corp., Irvine, CA, USA) was inserted in the external jugular vein and advanced until a satisfactory pulmonary artery occlusion pressure was obtained.
For interlaminar injections, a Tuohy needle was inserted in or near the midline and advanced into the epidural space with image guidance in the anteroposterior and lateral views by using the loss of resistance technique.
A pulmonary arterial catheter (Edwards Lifesciences™, Irvine, CA, USA) was inserted in the right jugular vein and advanced into the pulmonary artery for measurement of mean pulmonary artery pressure (MPAP, mmHg), pulmonary capillary wedge pressure (PCWP, mm Hg) and cardiac output (CO, L/min).
