Arterial blood glucose was measured using an indwelling catheter inserted for hemodynamic monitoring with the use of a hand-held blood glucose meter (Accu-Chek Performa, Roche Diagnostics France, Meylan, France).
In addition, ChAd vectors have a large insert capacity (~8 kb), achieve high level, persistent transgene expression and are adaptable for use with diverse immunogens.
We used several secondary outcomes including infusion related cost, which was calculated in two ways costs associated with catheters inserted for the intermittent administration of intravenous drugs, and cost associated with catheters inserted for continuous infusion.
Sections were scanned using the Aperio ScanScope XT (Aperio, Brisbane, Australia) with doubler inserted for x40 scanning.
The Nup153FGPxFG fragment was modeled on the basis of its sequence that also included the exogenously inserted residues used for labeling of the fragment with fluorophores.
White colonies were picked for insert screening using PCR with M13F and M13R primers, and then grouped by RFLP with restriction enzymes RsaI and/or MspI.
To achieve our goal of a flexible, modular method for making fluorescent reporters, we designed a system that uses standardized marker cassettes that can be inserted in frame with a coding sequence for use in human or mouse cells (Fig. 1A C).
Transformants were grown on LB agar containing Kanamycin (100 μg/ml) and positive clones were confirmed for the right insert using PCR with primers (T3 and T7), which were complementary to the flanking regions of the PCR insertion site of the pCRTM4-TOPO® vector.
This tubular test assembly incorporates ten rigs of about 200 cm length used for inserting instrumented test capsules with miniaturized specimens taking advantage of the 'small specimen test technology'.
