Full-length sequences in this dataset have been inserted into expression vectors previously for expressing recombinant proteins in E. coli with the GATEWAY tags (data not shown).
Then the LAH-HBc gene was inserted into expression vector, yielding recombinant vector pET28a-LAH-HBc, and the expression was carried out in E. coli BL21 (DE3) strain.
The paper later disclosed that presidential aides had inserted his expression of regret after the fact.
Similarly, the fadR gene amplified from S. oneidensis was inserted into expression vector pET28(a), generating the chimeric plasmid pET28-fadRshe (Table 2).
By inserting a codon optimised Campylobacter oligosaccharyltransferase onto the E. coli chromosome, we created a glycoprotein platform strain, where the target glycoprotein, sugar synthesis and glycosyltransferase enzymes, can be inserted using expression vectors to produce the desired homogenous glycoform.
So in this study, fusion genes of h-UTI and domain I, domain I and domain II, domain I, domain II and domain III of human serum albumin were inserted into expression vector pPICZαA, respectively.
So in this study, fusion genes of h-UTI and domain I, domain I and domain II, domain I, domain II and domain III of human serum albumin were inserted into expression vector pPICZαA, respectively (As shown in Figure 1).
Different fusion genes of h-UTI and domain I, domain I and domain II, domain I, domain II and domain III of human serum albumin (HSA) were inserted into expression vector pPICZαA.
The codon-optimized gene was inserted into expression cassette pPJV7563 as described for pPML7800.
An amplimer encoding the entire Clect27 coding sequence were inserted into expression vector pET-22b (Novagen) between the NdeI and XhoI sites.
