Dense angiogenic sprouting occurs from arteriovenous loops (AVLs) incorporating autologous vein grafts inserted into empty plastic chambers in vivo.
Foam specimens were prepared using the powder metallurgical route, inserted into empty thin-walled tubes and subjected to quasi-static and dynamic bending loading conditions.
The different retroviral packaging cells expressed retrovirus encoding either a validated dominant-negative (DN) mutant of calpain-I [8], wild type (WT) calpain-I [8], or no insert (empty vector).
Eight µg of plasmid DNA, containing either no insert (empty vector), wild-type c-CBL, S80N/H94Y c-CBL, Q249E c-CBL or W802* CBL was used for transfection in a 6-well culture plate.
PT67 retroviral packaging cells (Clontech, Palo Alto, CA), which express the 10A1 viral envelope for production of amphotropic virus, were transfected with pLNCX2 vector containing DN calpain-I, WT calpain-I, or vector without insert (empty vector).
(A ) WT, Gal-YAE1 and Gal-LTO1 cells were transformed with 2µ vectors containing either no insert (empty) or genes encoding human YAE1D1 and/or ORAOV1 as indicated.
In addition, plasmid pBBR1MCS5 without the insert (empty vector) was also transferred into the mutant and wild-type strains via conjugation with WM3064.
HEK 293T cells were co-transfected with the expression vector MDH1-PGK-GFP 2.0 containing either miR-106b~25 or no insert (empty control) and the pCL-Eco viral packaging vector in a 2 1 ratio, using the calcium phosphate transfection method.
So, Dh5αF′ cells bearing either modified M13cpSBP or unmodified M13cp helper plasmid were cotransformed with pspB carrying UH-RA.21 and pspB phagemids (Somers et al. 2011) without insert (empty phagemid) and plated on 2×YT plates containing chloramphenicol (Chlr) and Amp.
You insert an empty cigarette casing into a hole, as if you were sharpening a pencil.
