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Pyrene labels in the form of ethynylpyrenyldeoxyuridine have been inserted efficiently into oligodeoxynucleotides probes using phosphoramidite chemistry.
We describe the design and properties of a pyrene-labeled deoxyuridine that can be inserted efficiently into oligodeoxynucleotides using phosphoramidite chemistry.
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But these oligomers require other receptors to insert efficiently into the membrane, since insertion into BBMVs is highly efficient when compared with insertion into synthetic liposomes where insertion is inefficient (compare Figures 3A and 4A).
In this paper, the proposed radio resource allocation algorithm, GRAMAKA, for uplink bandwidth allocation is used to insert efficiently the slot of the resource block in UL-MAP as efficiently as possible.
Here, we used a Xenopus laevis oocyte microinjection assay to show that group II intron RNPs can insert efficiently into a plasmid target site in eukaryotic nuclei, but require the injection of additional MgCl2.
This feature together with their high insertion frequencies and specificity enabled the development of mobile group II introns into novel bacterial gene targeting vectors ("targetrons"), which can be programmed to insert efficiently into desired DNA targets simply by modifying the intron RNA [8].
A "mini-CD4" (mcontainingining only these domains was constructed (Table S2) and verified to insert efficiently and correctly into ER microsomes by in vitro translation.
Thus pre-pore structures formed outside the bilayer require other proteins to insert efficiently into the membrane, such as has been proposed previously for APN or ALP receptors [ 8, 9], supporting the sequential binding model of toxin pore formation [ 12].
First, when the activated Cry1Ab toxin was incubated in solution with cadherin in the absence or presence of trypsin, the oligomer that is formed in solution did not insert efficiently into the SUVs.
It is important to note that oligomeric structures formed in solution from activated toxin were able to insert efficiently into BBMVs isolated from M. sexta as shown in Figure 4(A).
To date, while many studies have shown that lentiviral vectors containing miRNAs can transduce mammalian cells and express the inserted miRNA efficiently, no study has examined the impact on the replication of a lentivirus such as HIV-1 after the deliberate intragenomic insertion of a bona fide miRNA.
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