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The extra atomic planes are inserted as indicated by arrows.
Subdermal needle electrodes were inserted as indicated above.
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Transformation of Arabidopsis with 35S::CEL9B3SP: ScAGU115 resulted in 15 independent homozygotic lines that contained a single insert as indicated by the segregation analysis in T2, and 14 of those lines expressed the transgene, as found with RT-PCR (Additional file 2: Figure S2A).
Statistical significance was assessed as indicated.
Test, publish, review and change as indicated.
The vector for expression of GST-elafin (a.a. 61 to 117) was obtained by PCR amplification of the DNA coding sequence of trappin-2 (pre-elafin) with specific primers and using the pGE-SKAP plasmid (gift of Thierry Moreau, Tours, France) as a template, and the PCR product was inserted into pGEX4T-1 as indicated above.
The X indicates the position of the inserted stop codon, as indicated on the sequence alignments in Fig. 2 (A).
Our review of 228 dual-mobility cups inserted as THA revision components indicates a relatively low frequency of re-revisions due to dislocation within 2 years, although all the patients in our cohort were revised due to dislocation of the previous THA, and 25% of all the patients had undergone 1 or more previous revision surgeries.
The 5c-g insertion appeared to be specific to Saccharomyces and indicated that at some point during evolution this piece of the RNA was inserted near the 5' end as indicated in Fig. 4. The evolution of Saccharomyces also involved the enlargement of helices 5h and 5i.
It inserts by homologous recombination, as indicated by the absence of target site duplication, and causes rearrangements.
Cohesive zone elements are inserted at the crack path, as indicated in Fig. 6.
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