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When Alu with poly-A are inserted as above, it is in the double helix form with the complementary poly-T.
For mammalian assays, the pACYC backbone was first modified by including an RsrII site upstream of the PciI site, and then Tev-mTALEN constructs were inserted as above.
For hKIF15-eGFP an AscI-site was introduced in front of the NotI-site and the eGFP inserted via AscI/ NotI before the hKIF15 ORF was inserted as above.
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Vascular catheters were inserted as described above, 8 days prior to the clamps to allow for surgical recovery prior to minipump implantation.
Subdermal needle electrodes were inserted as indicated above.
The right tibia had a titanium screw inserted as described above.
The whole CSR1 gene for the gene reconstitution was amplified using the primers CdCSR1-1 and CdCSR1-5, the ApaI/BglII-cut DNA fragment was integrated into pSAP2KS1 (30), and the CSR1 downstream DNA element was inserted as described above.
Carlos Delgado was inserted as the second baseman.
are inserted as ordered triplets.
Groups were inserted as criterion.
Eggs from the NL5901 strain of C. elegans were treated with alkaline bleach, washed, and then placed in 12 well cell culture inserts as described above in the presence or absence of βHB.
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