Sentence examples for inserted a sequence from inspiring English sources

To enable efficient detection of both P3 and P3N-PIPO with the same antibody, we inserted a sequence encoding the V5 epitope to tag both the P3 and P3N-PIPO proteins near to their N-termini (Fig 1).

In this cassette we inserted a sequence coding for an HA tag in front of the LRRK stop codon and flanked the construct with an I-SceI and an I-CreI endonuclease site as well as with attB sites on either side.

When it concludes that some place is not a breakpoint, but should instead contain an insertion, it inserts a sequence of Ns.

Next, they introduce a few mutations into its genetic code — perhaps by inserting a sequence from another enzyme with different desirable properties.

Improving DNA vaccines by inserting a sequence involving NKG2D receptor-ligand interaction was shown to enhance innate and adaptive immune responses [36].

The pGEX-loxP plasmid was generated by inserting a sequence containing the loxP recombination site and bacterial promoter, for Cre recombination and an antibiotic selection gene, respectively (sequence information is available in The Creator Cloning System Manual, Clontech), into pGEX-4T-1 (atersham) athehEcoRIRI and XhoI sites.

Alternative splicing may affect the protein sequence in two ways: (i) by deleting or inserting a sequence and creating long and short isoforms, or (ii) by substituting one segment of the amino acid sequence for another [ 11].

Then, if D n,m) is smaller than the median value, we insert a sequence composed of letter 'N' , the number of which is decided by comparing D n,m) and the median intron size.

The introduction of fast acute loss-of-function systems for lncRNAs, for example those that insert a sequence-specific ribonuclease site whose nuclease is under drug inducible control, would also greatly facilitate lncRNA investigation.

Laising Yen, a postdoc in Mulligan's lab, and colleagues inserted a ribozyme sequence into a gene that coded for an easily detectable protein.

Similar to prestin bait, we inserted a partial sequence of cdh23 cDNA into the bait vector pTMBV4 through an in vivo recombination method.