Sentence examples for insert yielding from inspiring English sources

To assess the frequency of MVT in an S. cerevisiae library generated using a standard protocol [ 12], freshly prepared electrocompetent yeast were transformed with 1 μg of vector backbone and 5 μg of mutagenized DNA insert yielding library H-1 5H-1 5

To assess the proportion of MVT in yeast libraries generated using similar total plasmid concentrations, site-directed lysozyme gene libraries were constructed using the ISOR strategy [ 11], and freshly prepared electrocompetent S. cerevisiae were transformed with 500 ng of vector backbone and 1.25 μg of gene insert yielding library L-1 2.5. L-1 2.5

Out of the total reads, any reads without a perfect 10-nt linker subsequence were directly discarded adjoining the insert, yielding of length 10 nt or longer that were subject to further processing.

For that purpose, we identified the site at which a loop containing the recognition sequence for cre-lox recombinase could be inserted yielding a functional enzyme.

Coverage recovered further upstream as a function of insert length cutoffs, with longer library inserts yielding larger depletion zones.

In a second experiment, the complete ywpE gene including its start codon was amplified using the ON61/ON62 primer pair and inserted into pMUTIN4 yielding pNDH27.

For the simulation, the pilots are evenly inserted every eight subcarriers, yielding P=8.

The upstream and downstream sequences of the nprE gene were inserted into plasmid pUK, yielding nprE knockout vector pUNprEK.

Finally, the antibiotic-resistance marker Spec was amplified from plasmid pIC333 and inserted into vector pGAprEH, yielding integration vector pGAprEHS.

PCR products were inserted into vector pcDNA3.1 zeo+), yielding recombinant plasmid pcDNA3.1-huPrP-3AV.

This implanted motif was inserted every 10 seconds, yielding 171 motif-repeat pairs.