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The colonies were screened for plasmids having the appropriate insert by restriction digestion.
The peripherally inserted central catheters were donated by the AngioDynamics Corporation without restriction.
For improved data quality, vector bands, clones without inserts, and restriction profiles with less than 20 or more than 200 bands were removed and the remaining profiles were uploaded to FPC v8.5.3 [ 25] for fingerprint contig assembly.
The latest ligation-independent cloning, such as CloneEZ and In-Fusion cloning kits, uses some DNA polymerase to generate sticky ends in the vector and insert without specific sequence requirement, except for restriction sites to linearize the vector.
Plasmids were checked for inserts by restriction digest at the multiple cloning site.
It is freedom without restriction.
Data are freely available without restriction.
Smugglers operate without restriction inside Idomeni.
He could do his job without restriction.
These records are open for research without restriction.
All date are fully available without restriction.
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