Your English writing platform
Discover LudwigSuggestions(1)
Exact(3)
F. Insert Transfer Tube pre-loaded with HDR fluid into EpiCollector.
It remains unclear whether or not specific entry vector/destination vector recombination reactions might affect the efficiency of insert transfer, but this will need to be tested by the end user on a case-by-case basis.
With TPS, a large majority of the cDNA clones constructed by using the Gateway-compatible cloning vector (pCMVFL3; Toyobo, Osaka, Japan) were sequenced by using a combination of TPS and insert transfer with Gateway technology to reduce the number of shotgun clones with transposons in the vector sequence [ 31].
Similar(57)
To confirm that the genomic gfp sequence had been converted into the yfp, PCR was conducted using primers (Table S1) specific for yfp and the 35S promoter driving the selectable marker gene (HPT), the latter expression unit being present within the stably inserted transfer DNA that also carries the gfp gene.
Culture medium was aspirated and the inserts transferred to 12-well plates containing 1.5 ml/well donor buffer (DMEM without phenol red, 25 mM HEPES and 0.1% bovine serum albumin) and placed in an orbital shaker at 37 °C.
All the workflows generated by W are then transformed to insert the transfer nodes whenever there is a inter-cloud edge in the workflow graph.
Additionally, each male was given up to 5 min to insert and transfer sperm.
Since sandwich panels are basically unsuited to carry localized loads, the sandwich structure should provide joining inserts to transfer the localized loads to other structures.
The insert was transferred to a clean well containing Cell Stain Solution for 10 min and washed three times in water.
Each insert was transferred in Petri culture dishes (Corning, Lowell, MA, USA) containing 14 ml of the EpiLife medium with HKGS, KGF (10 ng/ml) 1.5 mM calcium, and 50 g/ml vitamin C. Cells were incubated for another 13 days to obtain stratified cultures with the differentiation characteristics of a human epidermis.
The sequenced insert was transferred to pMDC32 and pCAMBIA1305.1 [ 35] using the Gateway LR Clonase™ II Enzyme Mix (Invitrogen).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com