Sentence examples for insert together with from inspiring English sources
After briefly vortexing, 300 µl of solution were transferred to a small insert together with 600 µl ddH2O.
Their insertional nature and position was determined by detection of sequences homologous to the reference genomes at the ends of the scaffolds and reads containing part of the sequence of the insert, together with part of the reference genome sequence.
One microliter of each concentrated cDNA insert together with one microliter of a 2 ng/μl solution of amplified nebulin were transferred onto 8 × 12 cm Hybond+ nylon membranes (Amersham, GE Healthcare Bio-Sciences AB, Little Chalfont, UK) using a manual 96-pin tool.
The restriction site in the 3' UTR into which the IRES-CreER/TetR/FNF cassette was inserted, together with the sequence of ten nucleotides on each side of the insertion sites are as follows.
The loxP flanked neomycin resistance - thymidin kinase gene (neo-tk) selection cassette was inserted together with a 141 bp wt cDNA fragment of the β1 integrin gene coding for the entire cytoplasmic tail of the β1 integrin chain including the stop codon, into exon 15, just behind and in frame with the transmembrane coding region.
The prostheses were then inserted together with cement and compressed axially.
Furthermore, the healing of endosseous implants was not improved when those were inserted together with OCHS [ 21].
The medium from the upper inserts, together with non-invading cells were removed off the upper Matrigel surface.
S. cerevisiae EBY100 (Invitrogen, Carlsbad, CA, USA) was transformed with the gel-purified library inserts together with BamHI/NotI-digested pYD1 using the lithium acetate method [7].
Inserts together with a 5′-T7 promotor sequence were amplified with M13 plasmid-specific primers and transcribed into RNA by use of Megascript T7 reagents (Ambion, Austin, TX, USA).
The next knotless anchor was then inserted together with the tape through the infraspinatus tendon and into the valley of the Hill-Sachs defect.
