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Homologous recombination of the 82 kb overlapping region and the common sequence of the BGM vectors resulted in the extension of the insert to generate Tg-250SB.
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With bibloc devices, the lateral attachments producing mandibular propulsion can be inserted to generate traction or compression.
By adopting a traditional generation method for the coastline echo under small radar range, the time for the scan line to rotate around is much more likely to exceed three seconds, since extra sampling coordinate points (if available) have to be inserted to generate a high quality coastline echo.
Into the MCS-B site, separated from the MCS-A by an IRES sequence, the DHFR gene was inserted to generate the pIRES-DHFR vector (Fig. 1A).
To provide chemical specificity, protoporphyrin IX (PPIX), the penultimate molecule in the heme synthetic pathway into which a ferrous iron is inserted to generate heme, was used to increase heme synthesis[34].
Then, GFP and SV40 terminator sequences were inserted to generate pBacGFP-3xP3DsRed.
The GFP cassette of FUGW was removed and mCherry cDNA inserted to generate FUmChW.
mCD8 coding sequence (Lee and Luo, 1999) fused to mCherry or GFP was inserted to generate pKMC191 or pKMC192, respectively.
A 5.2-kb KpnI-XhoI genomic subclone was inserted to generate the left arm of the targeting vector.
Briefly, cells were plated 24 h after transfection onto 24-well plates that contained inserts to generate defined 'scratch areas'.
Similarly, the phl-et cassette, a NotI fragment from pBEAZ195 [ 24], was inserted to generate the plasmid p108Phlet.
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