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The entire protein coding region of EGFP-pDisplay was then PCR amplified and cloned into a pEGFP backbone lacking the EGFP insert, thereby introducing a second, distinct, intracellular multiple cloning site (MCS-2).
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Importantly, bar-codes can be directly incorporated onto the primers used to amplify the prey-plasmid inserts, thereby permitting the pooling of independent samples prior to Illumina sample prep.
There could also be a bias for the insertion of retrogenes in the same regions where retroelements insert given that they are known to use the same machinery [ 28] or for both of them to insert in open chromatin, inserting thereby in the same regions [ 29].
Genomic clones that hybridized to the sd1 or rht1 probes were digested with NotI to identify clones with a greater than 4-kb insert size, thereby potentially carrying a full-length gene.
In addition, these plates can be inserted percutaneously thereby decreasing soft tissue injury and leaving a better cosmetic result.
Known insert size, and thereby a known distance separating the paired reads obtained, provides additional information for later assembly or mapping [ 8].
If mutations in the organellar genome for example changed the properties of the OM pores and made it easier for the host to insert carriers and thereby increase the fitness of the consortium, such mutations could have spread.
The compliant element is thereby inserted between a brushless direct current motor, a gear reduction and the load.
This PhC structure was conceptually separated by inserting a gap, thereby creating a microcavity - but the Q-factor value for this device was only 500.
Stability can be engineered into IMPs by inserting stabilizing mutations, thereby generating proteins that can be successfully applied to biochemical and structural studies when solubilized in detergent micelles.
Using this strategy, one end of all inserts was identified thereby enabling the relative positions of all fragments to be deduced.
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CEO of Professional Science Editing for Scientists @ prosciediting.com