(A ) Diagram of the Nrp1 genomic region following successful homologous recombination to insert the targeting vector.
To develop a simple approach for indicating the target protein expression of E. coli in real-time, we engineered a short ORF containing MCS for inserting the target gene, which was placed upstream of another ORF encoding the reporter fluorescence protein.
Also, inserting the target sequence of host plant's miRNAs in the virus genome can impair virus infectivity; however, the virus can escape rapidly of the miRNA action by mutations [ 24].
Plasmid pSuper-shESE-1, which was used to generate siRNA against ESE-1, was constructed by inserting the target sequence into pSuper (Oligoengine, USA) in site with Bgl II and Hind III.
This type of mutually exclusive folding can be achieved by inserting the target protein sequence into a surface loop of the control protein so that the attachment points are too close together for the target protein to adopt its normally folded conformation unless the control protein unfolds, or unless it undergoes a domain swap.
The data presented to implicate direct carbamylation of the connexin protein (at K125 in Cx26) are compelling, given that CO2 induced opening of hemichannels is eliminated using mutants at this site, and can be introduced into a non-CO2 sensitive Cx31 by inserting the target sequence.
pGreen-FRS2 α, pGreen-Mcl-1 and pGreenPuro-vec constructs, encoding shRNA for FRS2 α (sh-FRS2), Mcl-1 (shMcl-1) or a negative control (vector) respectively, were prepared by inserting the target sequence for human FRS2 α (shRNA1: 5′-CCGTGATAGACATCGAGAGAA-3′ or shRNA2: 5′-CCGTGCAGAAGAATTATTT-3′) or Mcl-1 (5′-GGACTTTTATACCTGTTAT-3′) into pGreenPuro.
Train the bird inside its cage by inserting the target stick into the cage.
Because hemifusion requires that fusion peptides of 3 4 adjacent trimers insert into the target membrane, the apparent rate constant for hemifusion relates to a different probability that of inserting fusion peptides from several neighboring HAs.
Interestingly, only C8 alpha-gamma and C9 insert into the target cell membrane (reviewed in Dunstone and Tweten, 2012).
