The rats were then injected with the Tf-TR solution by inserting the injection needle 4 mm deep into the CNS lateral ventricle.
After the turning process, the metal sheet is striped off and inserted in the injection mold.
The fiber was inserted into the injection port of the GC at 240°C.
Both the sample and the washing solvent are transferred into the split-injection port through a narrow bore fused silica capillary inserted into the injection liner through a septum.
The fiber was exposed to the vapor phase for 5 min to sample the volatile compounds, and then inserted into the injection port of the GC-MS (GC QP 20120, Shimadzu) using an inlet liner (0.75 mm i.d).
The SPME fiber with absorbed volatile compounds was inserted into the injection port of Thermo-Finnigan Trace GC/MS (Thermo Electron, San Jose, CA) system and desorbed for 5 min at 230°C.
The SPME fiber was exposed to each sample for 10 min by manually penetrating the septum, and, finally, the fiber was inserted into the injection port of the GC for 10 min sample desorption.
The SPME fibre with absorbed/adsorbed (depended on the fibre coating) volatile metabolites was inserted into the injection port of an Agilent Technologies 6890N Network gas chromatograph system (Palo Alto, CA, USA), and metabolites were desorbed for 6 min at 250°C.
During the operation, wound secretion was obtained by a syringe inserted close to the injection site.
It could be done via the cystic duct using a percutaneously inserted catheter with the injection of an contrast agent.
