Sentence examples for insert the entry from inspiring English sources

Some Destination vectors insert the Entry cassette in their 3'LTR (see below), but this imposes an insert size limit, usually ∼800 bp [37], because larger inserts inactivate the LTR and impair viral integration.

The insert of the entry vector was subcloned into the destination responder vector by LR-recombination using the gateway recombination system in order to obtain a protein expression vector [43].

The insert from the Entry vector clone can then be recombined efficiently via an attL-attR (LR) reaction [17] into a variety of Destination vectors to provide the desired viral backbone and drug selection marker.

After confirmation of DNA sequence, the insert of the entry clone was transferred to pAS-GW using LR recombination reaction according to the manufacturer's instructions.

The three PCR fragments were inserted into the entry vector pENTR-D-Topo (ceQORH, AtHMA1) and pDONR221 (AtAATpDONR221

To create the expression constructs, the cDNA inserts of the entry clones were shuttled into the pEF-DEST51 destination vector (Invitrogen; 12285-011) using LR-clonase recombination (Invitrogen; 11791-020) according to the manufacturer's instructions.

Therefore, as shown in Figure 3, we also inserted in the entry all possible tryptic peptides from the position -25 up to +7 of the predicted site, to achieve a better coverage of possible positions of N-terminal cleavage.

Therefore, this peptide is re-inserted in the entry after a J letter (box) and Mascot trypsin parameters are changed to consider the J code as a cleavage site of trypsin.

Inserts cloned into the entry clones were subsequently recombined into the destination vectors via an LR reaction to create the expression constructs.

Investigators said the victim, Burke O'Brien, had stepped out of a cab with a male friend at 4 a.m. and was inserting his key in the entry lock of the six-story walkup at 75-79 Orchard Street, between Grand and Broome Streets, when the assailant shot him once in the chest and fled.

After clamping the catheter, a 21G needle connected to a pressure monitoring set was inserted and secured in the entry point of the catheter.