When the substitutions were inserted in the context of a larger promoter, also containing part of the ubiquitous minimal promoter (S -400/+259), no change in activity was observed for either.
To use fastFISH, the three-base target sequence must be inserted within the context of an existing four-base (i.e., 'structured') RNA sequence, which, in principle, might sequester the fastFISH target from hybridization.
And because they fail to insert themselves into the context of what matters most to their audiences, they miss the big chance to communicate true value.
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The Ad5 fiber knob domains (GenPept: AP_000226.1) with peptide insertions (scramble, GE11, M*, and LS) were modeled by using SWISS-MODEL software 28 30 (Basel, Switzerland) and edited in PyMol Molecular Graphics software (version 1.1eval, Schrödinger, LLC, NY) to assess the spatial conformation of the inserted peptides in the context of the homotrimeric knob structure.
This fragment may prove useful in reducing the insert size further in the context of a dsSINV vector.
In the second OIC sequence the animals that had received Mb as the context insert in the first sample phase and Dw in the second sample phase continued to do so, as did the animals that had experienced the reverse.
For the first OIC sequence half of each of these groups (ns = 4 and 3) had Mb as the context insert in the first sample phase and Dw in the second sample phase; this arrangement was reversed for the remaining rats.
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Previous studies have inserted the term 'synergy', in the context of miRNA regulation, to describe pairs of miRNAs that significantly co-regulate at least one functional module [ 70].
