Sentence examples for insert the addition of from inspiring English sources

Due to the length of the insert, the addition of a βgal expressing cassette (4.5 kb) reduces to the minimum the probability of reversion to wild type forms of the BoHV-1.

Concurrently, CS-PEO/Fb scaffolds were shown to maintain their substructure for a minimum of 48 h (Fig. 10: insert), and the addition of 8 μg/mL of PDGF did not alter the scaffold degradation properties.

With an umber codon inserted during the addition of the FL tag and CloveDirect TAMRA (6-carboxytetramethylrhodamine; Protein Express), the pinpoint fluorescent labelling protein to which TAMRA-aminophenylalanine was introduced into the ninth amino acid was produced.

In addition, the long terminal repeats (LTRs) were removed and transcription of the VLP insert was driven by the addition of the cytomegalovirus immediate-early (CMV-IE) promoter.

When these PCR fragments were inserted, we found that the addition of the amino acids RA to the truncated mRFP1 yielded the brightest fluorescent colonies (Fig. 2B).

The inserts were placed (before the addition of cells) in wells containing DMEM high glucose supplemented with 10%% FBS.

Despite the thickness of the culture plus insert at around 150 μm, the addition of this neutralizing antibody abolished both the down-regulation of ZO-1 protein and increased vascularisation induced by in vitro seizures [ 21].

For protein expression in Escherichia coli, constructs were transformed into BL21-DE3, grown in LB supplemented with kanamycin (20 μg/ml) to an OD of 0.6, and T7-driven expression of insert proteins was induced by the addition of IPTG to a final concentration of 200 μM.

Once parasites containing these inserted promoters have been cloned, the addition of ATc would switch off expression of the targeted gene, producing a rapid and observable phenotype if the gene is essential.

This effect was almost totally abrogated when contact between the two cell types was prevented with 0.4 μm transwell inserts or partly prevented by the addition of antibodies against β2 integrins or of anti-IL-15 mAbs.

Finally we analysed whether the enzymatic activity is competitively inhibited by the addition of the insert in trans.