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Low quality reads, reads without the adaptors, reads with polyA sequences and reads without the insert tag where removed.
After excluding low quality reads, reads with 5' primer contaminants, reads without 3' primer, reads without the insert tag, and reads shorter than 18 nt were removed.
After removing low quality reads and reads with 5′ primer contaminants, reads without 3′ primer, reads without the insert tag, reads with poly (A), and reads shorter than 18 nt, the clean reads were obtained.
In this step, clean data were obtained by removing reads containing more than three N (undetermined bases), with 5' adapter contaminants, without 3' adapter or the insert tag, containing poly A or T or G or C and low quality reads from the raw data.
The raw reads obtained from the Solexa sequencing were cleaned by removing adaptors, low quality tags as well as contaminant reads including those reads with 5´-primer contaminants, reads without 3´-primer, reads with poly A, reads without the insert tag, and reads with length less than 18 nt.
According to the requirement of this experiment and the principle of bioinformatics analysis, some contaminant reads should be removed from the raw reads, such as low quality reads and reads with 5' primer contaminants, reads without 3' primer, reads without the insert tag, reads with poly (A), and reads shorter than 18 nt.
Similar(53)
During this procedure, all low quality reads were removed, such as reads with 3′ and 5′ adapter contaminants, those without insert tags, and those with poly A sequences.
The validated report containing mapped entities is then used to automatically insert tagged values for the semantic concepts back into the XMI representation of the UML model.
The reads shorter than 18 nucleotides, containing 5′ primer contaminants, containing poly A tail or missing 3′ primer, and insert tags were also excluded from the data sets.
The workflow for obtaining 'clean' reads involved filtering low quality tags; removing raw reads with 5' primer contaminants; trimming 3' adaptors; removing reads without insert tags; discarding reads with polyA tails; and removing contaminants formed by adaptor adaptor ligation.
After discarding the sequences shorter than 18 nt, eliminating low-quality sequences and removing contaminants formed by adapter adapter ligation, reads without 3′ ligation and insert tags were obtained.
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