Exact(6)
In addition, the model of direct coculture used in the present study could be considered to be closer in a way to the physiological context compared to indirect coculture using previously described cell culture insert systems, where trans-differentiation events were observed [15], [21].
Only a few inverted culture insert systems exist so far that enable investigation of leukocyte transmigration across cellular barriers.
Calu-3 cells as well as primary human ATII cells were also previously used in inverted culture insert systems [ 34].
Trans-well experiments were performed using 8.0-um pore size 24-well insert systems (BD Falcon) with 2 mg/ml of Matrigel coating (invasion) or not (migration).
Scanner designs allowing simultaneous PET and MR acquisitions (insert systems and fully integrated systems) may help to keep scan times shorter for the patient.
Note that conventional cell culture insert systems with a semipermeable membrane are not suitable because their pore size (≥0.4 μm) allows the free passage of glucose oxidase and catalase molecules.
Similar(54)
Håkan Johansson and Petter Sjödin of Composult Composite Consulting AB explain how a non-destructive, post-fitted insert system enables you to cut manufacturing costs, minimise weight and simplify design.
Due to the insensitivity of the SiPMs to magnetic fields and the compact front-end electronics, the detector approach is highly attractive for developing a PET insert system for a magnetic resonance imaging (MRI) scanner to provide simultaneous PET/MR imaging.
A 0.4 µm cell culture insert system was used for the indirect coculture assay (BD Biosciences).
CMFDA-labeled cells (105) were added to each insert of 24-Multiwell plate (BD Falcon™ HTS FluroBlock™ Insert System, 8-µm pore size), and 1 ml of phenol red-free αMEM with increasing concentrations of SDF-1α (PeproTech) or fractalkine (PeproTech) was added to the lower compartments.
Falcon™ cell culture insert system was from Becton Dickinson and Company (Franklin Lakes, NJ).
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