Calu-3 lung adenocarcinoma cells were maintained on membrane inserts such that the basal surface was bathed in nucleophile-free media while the upper surface remained in contact with acrolein-containing air.
Since the most N-terminal protein has the best opportunity for proper protein-folding and proper folding of our reporter fluorescent protein is imperative for efficient screening, another method to enhance on-plate fluorescence screening is creating gene inserts such that its integration will reconstitute a truncated N-terminal fluorescent protein.
Under visual control, the catheter was inserted such that the inflated balloon was clearly located within the jugular vein.
The targeting vector was inserted such that exons 3 5 were deleted, supposedly inactivating the gene and inserting a LacZ cassette containing a strong splice acceptor.
All grafts were measured and inserted such that the articular surface of the graft edges was flush with the articular cartilage of the recipient site.
During construction of the pRS31x plasmids (Sikorski and Hieter 1989), the authors had intended for the CEN6/ ARSH4 cassette to be inserted such that CEN6 would be closest to the bla gene, but CEN6 is instead closer to the HIS3 marker in both pRS313 and pRS413.
Consistent with this observation, in the one case in which it has been examined (large-conductance ion channels in the nuclear membranes in Purkinje neurons), the channels in the inner and outer membrane are inserted such that in each membrane they have the same orientation relative to the lumen (i.e., they "point" exactly as do the arrows in Figures 3, 4 and 5 ) [ 304 ].
Several European countries, supported by the United States, Tunisia, Kenya and others, managed to insert such language into the proposal, arguing that "nondiscriminatory access" to telecommunications was an important free-speech issue.
Sherwood claimed he did not know how much it was and did not agree when it was put to him that by inserting such a stipulation the club were leaving themselves vulnerable.
Participants were instructed to insert the probe such that the plate touched their labia.
