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Paired-end libraries (insert size of 200 bp) were sequenced with read length of 49 bp.
Integrative genome viewer snapshot shows reads paired in sequencing and sorted by insert size.
Figure 1 Detection of insert size.
Figure 2 Distribution of insert size.
The actual insert size excluding adapters was ~150 bp.
Average subread length can vary depending on insert size and DNA input quality.
End sequences from large insert BAC libraries in pBACehr (insert size 10 25 kb) and pBACe3.6 (insert size 12 15 kb) were used as a scaffold.
The draft phase involved two clone libraries, one small insert library (2200 bp average insert size) and one medium insert library (6289 bp average insert size).
Validating insert size.
Paired-end library (insert size in Kb).
Their average insert size was 1.2 kb.
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