Sentence examples for insert shotgun from inspiring English sources
Second, to estimate the abundance of Atlantys elements in the remaining species, we used Atlantys LTRs as queries in similarity searches against sequences derived from a set of random sheared small insert shotgun libraries generated from the 12 Oryza species under investigation (Table 2).
About 1,920 randomly picked fosmid clones were end-sequenced and the data pooled with the small insert shotgun sequence data.
Isolated DNA from S. cerevisiae S288c and CEN.PK113-7D CEN.PK113-7Ded to prepare short insert shotgun libraries at Fasteris SA (Geneva, Swereerland), utilizing the Solexa technology according to the manufacturer's recommendations (Illumina).
The genomes of E. pyrifoliae strain Ep1/96 and E. billingiae strain Eb661 were covered by short insert shotgun libraries with 1.5 and 2.5 kb inserts and fosmid libraries with 37 kb inserts (CopyControl™ Fosmid Library Production Kit, Epicentre, Madison, U.S.A).
On a magazine-fed shotgun like a Saiga 12 or Akdal 1919, the loading procedure is identical to that of a rifle - insert shotgun shells into the magazine and then insert the magazine into the shotgun.
The hierarchical genome-assembly process (HGAP) developed by Chen et al. generates de novo assemblies using PacBio sequencing data from a single, long-insert shotgun DNA library [28].
The high raw coverage afforded by the short-read technology minimized erroneous base calls and gaps in unique regions, whereas the longer inserts from the shotgun sequencing projects (3198-4789 bp inserts; trimmed reads averaged 179 bp in length) helped bridge repetitive regions and establish long-range scaffolds.
DNA isolated from the filter-trapped microbes was used to build small insert libraries for shotgun sequencing (Materials and Methods).
2. For each breakpoint: (a) A shotgun insert must span the corresponding fusion point.
As in the case of deletions, either breakpoint is detected when a shotgun insert of the query spans the corresponding fusion point, and has exactly one end-point inside the inversion.
Shotgun inserts in the pEZSeq™-vector were sequenced with M13_for: 5'-AGC GGA TAA CAA TTT CAC ACA GGand' and M13_rev: 5'-CGC CAG GGT TTT CCC AGT CAC GACAC'.
