Sentence examples similar to insert sense from inspiring English sources

"Everybody gets excited by this idea and they forget to insert common sense".

Only if inserted in sense into an active transcription unit do these TEs nucleate H3K9me3 spreading into the flanking genome.

The resulting fragment was inserted in sense orientation into the SnaBI site of the retroviral vector pBABE-puro.

The following adapter sequences flank the cDNA inserts: TrueSeq Sense primer 5′AATGATAC GGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3′ and TrueSeq Antisense NNNNNN primer (NNNNNN = 6n barcode for multiplexing) 5′-CAAGCAGAAGACGGCATACGAGAT-NNNNNN-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATC dT25 -3′ 5′-CAAGCAGAAGACGGCATACGAGAT-NNNNNN-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATC dT25 -3′ 5′-CAAGCAGAAGACGGCATACGAGAT-NNNNNN-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATC dT25 -3′ 5′-CAAGCAGAAGACGGCATACGAGAT-NNNNNN-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATC dT25 -3′ 5′-CAAGCAGAAGACGGCATACGAGAT-NNNNNN-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATC dT25 -3′

UKF-NB-4VCR cells were transfected using the calcium phosphate co-precipitation method with full-length cDNA, encoding the human NCAM-140 kDa isoform inserted in sense of the eukaryotic expression vector pHβ-Apr-1-neo [ 14].

The obtained fragments were subcloned and sequenced, and after excision with XhoI/ HindIII and ApaI/ BglII, respectively, inserted in sense and antisense orientation in vector pS-NAT1 (Janus et al. 2007).

Replacement of the GFP coding sequence by that of the HA epitope was made by digesting vectors pET-FPUG and pET-FPSG by BamH I and Not I and inserting annealed sense and antisense following oligonucleotides: 5'-GATCCTGTCTACCCATACGACGTCCCAGACTACGCTGGTAAGTAAGC-3'-GGCCGCTTACTTACCAGCGTAGTCTGGGACGTCGTATGGGTAGACAG-3AG-3'.

A loxP5171 flanked PGK promoter driven neomycin phosphotransferase gene (PGK neo) as a positive selection marker cassette harbouring a diagnostic EcoRI site at its 5′ border (pLoxPneo-2.5171, modified from plasmid pLoxPneo-1) (Nagy et al., 1998) was inserted in-sense into an artificial XhoI site in intron 18 of the NR1 gene.

NO. 14 CORNELL Insert "committee has sense of humor" joke here and pick the smart kids from Palo Alto.

"We're trying to insert Roz's sense of humor into the public sphere, but we want an element of surprise," said Mr. Klein.

Chris Peterman, a student at BJU (insert middle school sense of humor here), claims watching Glee on his computer at an off-campus Starbucks got him in big trouble with the school.