This was utopian fantasy, I thought — poachers would strip off any radio transmitter they found, and the microchips that it is possible to insert require a scanner to read.
In particular, restriction enzyme mediated cloning of an insert requires that the cloning enzymes do not cut within the insert.
In addition, Zhang and Huang did not include a selectable marker on the subcloning vector, but introduced it separately into the BAC insert requiring an extra recombination step and the subsequent removal of the selectable marker from the insert by frt recombination [ 8].
L. 110 315, § 403 e)(1)(A), (B), inserted "Required" before "services" in heading and, in introductory provisions, substituted "A project assisted under this section shall provide—" for "A postbaccalaureate achievement project assisted under this section may provide services such as—".
Histological staining of the membrane showed that the formation of monolayers on inserts required 6 × 104 cells and 5 days of culture.
The caucus's holdouts voted for the bill only after language was inserted requiring Mr. Rowland, a Republican, to rehire the roughly 2,800 or so unionized state workers he has laid off since December.
It was only in 1813, when the company's charter was renewed, that a clause was inserted requiring the governor-general to devote not less than 100,000 rupees annually to the education of Indians.
The methods for modifying BAC inserts require additional recombination components, e.g., RecA or phage-derived recombination proteins [ 3- 6].
The patient package insert is required to be placed in or accompany each package dispensed to the patient.
In addition, gene silencing of the PHYTOENE DESATURASE (PvPDS) marker gene was tested with recombinant BPMV vectors carrying fragments of increasing size to determine the minimum insert length required for efficient PvPDS silencing.
