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Colony direct PCR was performed for insert recognition using REDTaq (Sigma, St Louis, MO, USA).
Colony direct PCR was performed for insert recognition using REDTaq (Sigma).
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Sera samples, fully pre-adsorbed with wt-GV as described in the Methods, were assayed to detect SIV insert specific antibody recognition by using r-GV produced by the homologous transformants and the average absorbances at 405 nm were graphed.
EhSINE1s do not insert at specific recognition sites but have a preference for pyrimidine-rich regions [ 19]; Mandal et al. [ 11] have shown a strong preference for T-rich stretch of 15-20 nupstreames upstream of the insertion site.
Engineering viruses by inserting microRNA (miRNA) recognition elements (MREs) into the 3′-untranslated region (3′-UTR) of viral RNA can efficiently restrict viral tissue tropism.
In the only simulacrum of a platform fight this year, conservatives inserted language opposing recognition of "other living arrangements as equivalent to marriage," adding, just to drive the point home, that "legal recognition and the accompanying benefits afforded couples should be preserved for that unique and special union of one man and one woman which has historically been called marriage".
All vectors were modified by inserting two SfiI recognition sites into the multiple cloning site to facilitate transfer of coding sequences between vectors.
To overcome this limitation, we inserted FRT (flippase recognition target) sites flanking exons 2 6 of the endogenous p53 gene in mice to generate a p53 FRT allele that can be deleted by Flp recombinase.
Dual role of the nuclear factor of activated T cells insert region in DNA recognition and cooperative contacts to activator protein 1. Proc Natl Acad Sci U S A. 1997 May 13; 94(13):4913-24.
Israeli startup TicTacTi is looking to make monetizing casual games more efficient, by using image recognition to insert ads into casual gaming widgets.
Specifically, the diagnostic sequence elements for cDNA termini include adapter/linker sequences, insert-flanking restriction enzyme recognition sites, poly (A)/(T) tails, and plasmid vector fragments immediately adjacent to cDNA inserts.
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