Sentence examples for insert overnight from inspiring English sources

Ligation was performed between the digested p2845IFT140 plasmid and the TdTomato insert overnight at 16°C, the resulting plasmid was then transfected into XL-10 Gold Ultracomptent Cells (Agilent Technologies, France) to allow amplification.

Never leave a tampon inserted overnight; use overnight pads instead.

The cells were left to adhere to the inserts overnight.

The 16S rRNA gene sequences were determined by direct amplification of the inserts from overnight grown-clone cultures with M13 forward and reverse primers and sequencing with the DYEnamic ET Dye Terminator Cycle Sequencing Kit for the automated MegaBace 500 system (GE Healthcare), according to the manufacturer's recommendations.

Once a positive clone was identified, the insert was digested overnight with EcoRI together with empty pBABE-puro plasmid.

For the invasion assay, the insert was coated overnight at room temperature (RT) with 100  μl matrigel (50 ng ml−1 in PBS; Sigma Aldrich Chemicals, Zwijndrecht, The Netherlands).

The insert was coated overnight at room temperature (RT) with 100  μl matrigel (50 ng ml−1 in PBS; Sigma-Aldrich Chemicals, Zwijndrecht, The Netherlands).

For each cloning, eight colonies containing the tick DNA insert were grown overnight in LB medium supplemented with 10% glycerol and ampicillin (Teknova, Hollister, CA), frozen, and sent for sequencing to the HTGU.

In brief, the HT115 DE3) E. coli strain containing the pDP129.36 with a target gene-specific insert was grown overnight in 2× YT medium containing 100 µg/mL ampicillin (or 50 µg/mL carbenicillin and 12.5 µg/mL tetracycline) with stirring at 37°C.

For invasion assays, the procedure was the same as the migration assay except the 8  μm polycarbonate pore membrane inserts that were overlaid with (test) or without (control) 10 15  μg Geltrex (Life Technologies) and the inserts were dried overnight at 37°C prior to cell seeding.

The purified digests of plasmid and desired inserts were ligated overnight at 15°C using T4 DNA ligase, following the manufacturer's instructions.