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(2) Error-free bypass of the aldehyde adduct 1 (R = H) can also be achieved by the sequential action of TLS pols such that either Rev1 or pol ι will insert opposite the lesion, while pol ζ, pol η, or pol κ will extend (two-step, two-pol mechanism).
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An AR-coated MgF2 window and a glass slab were inserted opposite to each other as coupler and target.
This enzyme removes A incorrectly inserted opposite 8-oxoG.
However, if the O6MeG is not repaired before the re-synthesis step in MMR, the thymine is likely to be re-inserted opposite to the lesion.
In general, phase 1 mutations, located at the level of the U∶G mispairs, are only found after the replication fork has passed the abasic site produced by uracil-DNA glycosylase and a dNTP has been inserted opposite the abasic site.
We also evaluated extension past these lesions after a nucleotide has been inserted opposite these lesions.
Some truncated extension products were observed in both sequence contexts regardless of the identity of the base inserted opposite M1dG.
First, the mutagenic outcome of replication depends on both the identity of nucleotides inserted opposite the adduct and the relative efficiencies of extension from the products of insertion.
These results were consistent with the "type II" intermediate, in which the identity of the dNTP inserted opposite the lesion depends upon the 5′-neighbor template base.
The −1 deletion products are not as abundant in the 5′-C- M1dG -G-3′ sequence context because dGTP is not readily inserted opposite M1dG (Table 2).
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CEO of Professional Science Editing for Scientists @ prosciediting.com