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The MNO/PNO element derives from the assumption that if different siRNAs produced by a clone insert match sequences within the target transcript, then there will be a greater chance of the target transcript being knocked down compared with when siRNAs produced from a single region of a clone insert match only one or a few sequences within the target transcript.
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RNA probes were prepared from a cDNA insert matching the first 2975 nucleotides of the Arc mRNA (GenBank accession number NM_019361) cloned into the pCRII-TOPO vector (Invitrogen, Oslo, Norway).
Given that the BAC clone insert matches DXZ4 sequence at both ends, uninterrupted tandem arrangement of DXZ4 monomers should result in the generation of a predictable pattern of restriction fragments (Figure 2A).
When the sequence of the AVFV2 virus from the FV outbreak was analyzed, the 21-base insert matched perfectly with a region from the influenza matrix (M) gene.
You'll recognize those as having the familiar red and white plastic rings where you insert matching red and white plugs red for the right channel, and white for the left.
The restriction map of overlapping miniBAC inserts matched perfectly those predicted from contig sequences, demonstrating the reliability of the final contig assembly.
Sequencing analysis of the inserts matched exactly with the targeted gene sequences in the database (website: http://www.ncbi.nlm.nh.gov/blast).nh.gov/blast
Control and mutant mice were then assigned an insert to match a food reward in a counterbalanced protocol.
To assess the significance of any between-species differences, we extracted an equal number of random, paired B. tryoni sequences, with the insert sizes matching the gaps in the actual B. tryoni flanking sequence pairs.
Insert three matches -- wood-end first -- into one end of the flare.
Placing items that you don't offer year-round on a special insert (that matches the theme of the main menu) is cheaper than making multiple sets of menus.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com