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Segregation of the Kanamycin resistance phenotype was used to identify lines segregating for a single T-DNA insert location.
If care is taken to optimize construction of the four initial transgenic strains with regards to fitness, genetic background, insert location and fluorescence expression, rapid construction of an outcrossed MLA mosquito strain should be feasible because the desired genotypes can be automatically identified.
Depending on the insert location, mCherry-tagged fusion proteins may terminate at different positions along the peptide.
Several TEs are highly polymorphic for insert location in the genome and this facilitates development of TE-based markers for various genotyping purposes.
Report to the (insert location) at 1100-1400 (inserealeal time).
INSERT LOCATION HERE - DAY Write the setting and time.Depending on when you would like the action to unfold, it's advised to put day or night.
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You can get a list of locations (as well as insert locations) in both users' current location stream, as well as their historical stream (assuming they give your app permission to do so, of course).
Insert locations for most lines were determined by standard methods [35]; none of the isolated insert locations were within a coding region (Methods).
A confirmatory PCR with a GSP in combination with a T-DNA border primer (CMLB1 or CMLB2) was used to validate insert locations.
A single EcoR I recognition site is located near the left border of the inserted sequences, thus each band shown in Figure 2 should represent different insert locations.
Differences detected at the transcript level may be due to T-DNA insert locations, random mutations following transformation or direct effects of the recombinant protein itself, or a combination of these.
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