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A cardboard insert is added that says "U.S. Open '12" on one side.
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When the thickness of the tibial baseplate and insert were added to the tibial resection, the level of the dwell points of the insert averaged 3.6 mm ± 1.1 mm more proximal from the arthritic medial side (3.1 mm to 4.2 mm) and 1.4 mm ± 1.1 mm more proximal from the non-arthritic lateral side (0.8 mm to 2.0 mm).
Beginning October 16, 2009, a two-page "Bay Area" insert was added to copies of the Northern California edition on Fridays and Sundays.
M13_For: TGTAAAACGACGGCCAGT M13_Rev: CAGGAAACAGCTATGACC To each gDNA a trace amount of a plasmid with a unique non-feline insert was added.
MDA-MB-231 cells (2 × 10 cells per insert) were added to the upper chambers in serum-free medium, and 500 μl of RPMI medium was added to the lower chambers.
After plating epithelia into Matrigel stromal inserts were added to the cultures, media was replenished every 2 3 days.
One µl-aliquots of saturated E. coli cultures (DH10B) containing (pUC19 vector with random 3 kb DNA inserts or pMCL200 vector with random 8 kb DNA inserts) were added to 5 µl of a 10 mM Tris-HCl pH 8.2 and 0.1 mM EDTA denaturation buffer.
For basolateral infections (3 μm pore inserts), bacteria were added for 1 h (total volume 100 μl) to the porous surface of inverted inserts before re-inverting into fresh DMEM for continued incubation.
As described in the drug inserts, sucrose was added in 47.5% of the formulations, as well as citric acid (39.0%), sodium saccharin (36.4%) and sorbitol (34.8%).
Briefly, the Matrigel basement membrane matrix was thawed (4°C; overnight), the Transwell plates was put and inserted (both were added with serum-free medium) in a CO2 incubator (37°C; 2 h).
Bacteria are inserted and drugs are added to combat them.
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