Sentence examples for insert into a single from inspiring English sources

The second-round PCR is essentially the overlap extension PCR to assemble vector and insert into a single linear PCR product.

First some (rare) elements are site-specific, such as LINE R2 elements, which exclusively insert into a single site in the rDNA, or some IS elements in prokaryotes.

This DNA construct could then be used for mouse transgenesis to create an animal model where the multiple reporter genes would insert into a single locus, thus simplifying breeding schemes, yet expanding the capability and usage of the animal model.

A Southern blot analysis revealed that the T-DNA was inserted into a single locus in the alb1 mutant, so we performed TAIL-PCR to identify the T-DNA flanking sequences.

This entire process arises out of the genetic-like specification inserted into a single precursor cell.

Two 25 μm-diameter Teflon-coated wires [a] were inserted into a single 25-gauge hypodermic needle before sterilization.

A total of 4,927 inserted TEs were found to be inserted within 2,078 host TEs, thus revealing that >1 TE inserted into a single host TE.

Hundreds of pre-invasive lesions can be inserted into a single paraffin block, which can be sectioned and immunostained with a variety of markers [ 71].

To our knowledge, this is the first reported adenovirus-based, all-in-one Tet-On system with an autoregulatory loop inserted into a single region of adenoviral genome.

Both swabs are inserted into a single tube containing 3 ml of viral transport medium, which is labeled with a barcode; no personal identifying information is contained on the sample label.

However, in most cases, these comparisons were carried out after different IRESes had been inserted into a single, characterized, dicistronic (one IRES) or tricistronic (two IRESes) mRNA and after the in vitro vector translation efficiency had been established [ 9- 14].