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Using the recently solved structure of this domain [14], we demonstrated that the transport-deficient mutant M1046 harbours the linker insert in the first β-sheet, whereas both constructs displaying normal transport activity (L1192 and N1271) show linker insertions within loop regions (Fig. 6).
In the second OIC sequence the animals that had received Mb as the context insert in the first sample phase and Dw in the second sample phase continued to do so, as did the animals that had experienced the reverse.
One of these (Atsuc2-2) contained a T-DNA insert in the first exon, and the other two (Atsuc2-1 and Atsuc2-3) each had an insert at different locations in the second intron.
For the first OIC sequence half of each of these groups (ns = 4 and 3) had Mb as the context insert in the first sample phase and Dw in the second sample phase; this arrangement was reversed for the remaining rats.
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The mutants carry a T-DNA insert in the second intron of At5g40390.
This receptor is generated by intron 4 retention during RNA processing, resulting in a 69-amino acid insert in the third intracellular loop domain of the receptor [ 9].
Due to the reverse orientation of the inserts for nurf-1, the RT cassette was generated by two PCR reactions to add in a 50 bp region corresponding to the end of the insert in the second fosmid.
There are two splice variants of the D2 receptor, which differ by a 29-amino acid insert in the third intracellular loop in D2-Long (D2L) that is absent in D2-Short (D2S).
This receptor is generated by intron 4 retention during RNA processing, resulting in a 69-amino acid insert in the third intracellular loop domain of the receptor (Hellmich et al. 2000).
The T-DNA insertion of xylp16 allele represented in triangle inserted in the first exon (E1).
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