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Primers flanking the sequence of the small and large inserts of the rat myosin VI gene were designed and used to amplify the regions of interest [small insert: (forward: 5′-GATGAGGCACAGGGTGAC-3′ and reverse: 5′-GCGTATTTCCATTTACTGAGA-3′; large insert: forward 5′-GCTCCCAAGTCGGTTACT-3′ and reverse 5′-TTGTTCTGAGGGTCTTTGTA-3′)].
PCR analysis was employed to identify transformed colonies using primers directed against the I- κB α insert (forward: GCT GTG ATC ACC AAC CAG C; reverse: CTC TGG CAG CAT CTG AAG G) and for plasmid DNA for those containing pCMV4 (forward: GGT CTA TTC GGG AAC CAA G; reverse: CAC ATT CCA CAG AAG CTG C).
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At that point, Scott inserted forward Rodney Rogers for defensive matchup purposes.
Officials allowed Providence to replace him for the foul shots, and Coach Tim Welsh inserted forward Christopher Anrin, a 78percentt foul shooter compared to Gomes's 65percentt.
Set 1 was directed at the homology arms (forward: 5′-AGCCGGGGTCAGAGCACAGTAGTT-3′; reverse: 5′-ATCCATGAGCCCAAGCAAATCCT-3′), and set 2 was directed at PGK-NEO inserts (forward: 5′-GTCTGCCGCGCTGTTCTCCTCTTC-3′; reverse: 5′-CTTCGCCCAATAGCAGCCAGTCC-3′).
An expanding number of genes have also been identified and isolated after disruption or activation by Tos17, Ds or T-DNA inserts in forward genetics screens.
Thus, the theoretical yield for cells expressing in-frame peptides is 1/6 (the fragments can be inserted in forward and reverse directions).
Northern blot analysis showed that mRNAs from cells transfected with the renilla/firefly dfoxO 5'UTR-B inserted in forward orientation were similar in size to those with the dfoxO 5'UTR-B in reverse orientation (Fig. 3C).
While feeding from flowers, the labium is inserted straight forward into the corolla or in a slightly angled downward position (Johnson and Morita, 2006).
The DNA probes were amplified by PCR using primers flanking the cDNA inserts, the forward primer 5'-GTGTTGGTACCCGGGAATTCG-3' and the reverse primer 5'-GCTCGAGTCTAGAGTCGACTG-3'.
The recombinant clones having the specific insert in the forward orientation was then sequenced from both directions to get a contiguous sequence in an automated sequencer (ABI prism, model 377, Version 3.0).
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