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The sequences were almost identical; however, one clone had a 15 bp and another had a 19 bp insert exactly following the SL-sequence.
According to Ortega Hernández and Brena, Agnostus pisiformis does not show the dorsoventral mismatch [ 107], yet the specimen shown in plate 29, figure 4 of K. J. Müller and D. Walossek [ 108] clearly shows appendages to insert exactly under the edge-to-edge articulations of the tergites.
Similar(58)
It is important to note that, although ISPpu9 and ISPpu10 sequences are not highly similar, both are inserted exactly between the ninth and tenth bases of the REP sequence.
Make sure you insert it exactly in the middle.
Because there is exceedingly small likelihood that retroposons would be precisely excised or independently inserted into exactly the same orthologous site in different lineages, a retroposon insertion is a unique, powerful, nearly homoplasy-free phylogenetic molecular marker (Shedlock and Okada 2000).
Their contributions felt learned and rote, and it seemed not to be entirely their fault: they weren't used with much imagination, inserting solos exactly where you'd expect them, never locking into the music in any way.
P1-rw1077 and P1-rr contain the same MULE fragments and filler DNA inserted in exactly the same sequence position.
In fact, no intron (among fungal, choanoflagellate and ichtyosporean cox1 sequences) was found to be inserted at exactly the same nucleotide position as the predicted sponge intron.
Sequencing analysis of the inserts matched exactly with the targeted gene sequences in the database (website: http://www.ncbi.nlm.nh.gov/blast).nh.gov/blast
This intron occurs in psaA and is inserted at exactly the same site as the second of the two trans-spliced introns in Chlamydomonas psaA [ 30] (Table 3).
The positions of the inserts are not exactly the same although they are, indeed, very similar, and the stems in which the inserts are involved are imperfect.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com