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Exact(12)
The insert distance was about 200.
In all datasets, the error rate and insert distance were set to 1% and 250, respectively.
Moreover, only the components with consensus contig longer than insert distance are considered in this procedure, because otherwise a component caused by a repeat region shorter than insert distance will be connected to multiple components with enough supports.
A paired-end read represents two reads appearing in the same genome with known order and distance (insert distance).
Discordant pairs that mapped with an unexpected insert distance or orientation or to different supercontigs were identified and clustered to form regions of interest.
Non-coding genes were ignored, as well as 5′ reads that mapped to 5′-UTR, intergenic regions, introns or upstream of 3′-UTR, as the average insert distance between 5′ and 3′ paired end reads amounted to 180 380 bp.
Similar(47)
Assume the insert distances of paired-end reads satisfy the normal distribution N d, δ).
Newbler is able to sort contigs into scaffolds using the PEs but is often unable to incorporate conserved repeats into these scaffolds, which leaves gaps of approximated sizes based on paired-end insert distances (repeats are often assembled into "collapsed" contigs that remain orphaned after the assembly).
In principal, either the length of the fragment or the distance between the mutation and the CORE-GS insertion site (termed the "mutation-to-insert" distance) could influence incorporation frequency.
The current sequencing technologies are able to collect paired-end reads, where sequences of two segments are obtained separated by an approximate distance (insert length).
For mate-paired sequencing, the aligner software will attempt to match the two sequenced fragments within the insert size distance and orientation that is expected by the sample sequencing biochemistry and protocol.
Related(20)
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