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The library comprised all possible combinations of indices and candidate gene sequences (52 indices × 74 genes × 2 insert directions = 7,696 reference sequences).
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The selected plasmid DNA was digested by SacI to select the subclone in the right insert direction, and was then sequenced to confirm the right insertions without any secondary variants.
For crystal I, its insert direction is parallel to [1 1 0] and its extrusion direction is parallel to [¯111].
The two tectonic plates which meet at this fault line are heading in different directions (insert your own California joke here).
Each mutation was confirmed by sequencing the cDNA insert in both directions.
Successful cloning of the targeted gene sequence was then confirmed by Sanger sequencing the pRSETA-pvLDH 1-43aa insertwon two directions using vector and insert primers and an Abi 3100 genetic analyzer and recommended reagents and protocols (Applied Biosystems).
Because students produce clones with the 2.6-kb insert in both directions, there are two sets of mapping digests made and separated through the same large agarose gel, a total of 10 digests for each orientation of the insert.
There should be an insert slot with directions about orienting the check (face up or face down, etc).
If you do have the installation disk, follow these directions: Insert the installation disk and wait for the Setup program screen to begin.
(a) After 10 h of reaction, abundant nucleated AgCl cubic seeds appear, which began to show preferential overgrowth along <111> direction; insert is a growing 'flower' preferential growth from a cubic-faceted crystals seed.
The sequences were confirmed as correct by sequencing the inserts in both directions using standard T7 promoter and T7 terminator primers.
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